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  BLAST
- BLAST Tutorial
- About using tools
Query: Enter FASTA sequence
Alignment Options
General Parameters
Max target sequences:
E-value:  
Word size:  
Max matches in a query range:  
Scoring Parameters
Match/Mismatch Scores:
Gap Costs:
History

Previous result is not found.



  Phylogenetic Tree
- Phylogenetic Tree Tutorial
- About using tools
Select Accessions:
Hide
Show
Select Region:
Gene ID:    
Chromosome:  
Position:    -    
* Maximum size is 20,000 bp
Variant Filter: Quality:  >=  
DEPTH:  >=  
Note: It preferentially outputs variant type (ALT) allele regardless of its genotype "heterozygous or homozygous".
If GT is "1/2"(hetero without REF), the left side of the two alleles separated by comma is used.

OS01t0100100-01 Phylogenetic Analysis
Range of color gradient Color definition
SNP

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Min : ▓ 
INDEL

Max: ▓ 

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DEPTH

Max: ▓ 

Min : ▓ 

Max: ▓ 

Min : ▓ 
Note: The selected settings are applied to each block. If the minimum SNP and INDEL value is >1, the value is rounded-up to 1.
  Primer design
  • Run Primer3
  • Exporting sequence
- Primer design Tutorial
- About using tools
Settings
Target:
 :  -   
* Maximum size of  target + flanking region is 50,000 bp
Flanking region (bp):

  
* Maximum size of  target + flanking region is 50,000 bp
Avoid sites around
the target (bp): 		 
Variant sites masking:
Note: Variant sites ("hetero" or "homo") passing thresholds set below will be masked as "N" in the input sequence.
Quality: ≧  
DEPTH: ≧  
Avoid sites with
"Depth=NA":
Allowed ratio of "DEPTH = NA" accessions : ≤ 
Allowed number of "DEPTH = NA" accessions : ≤ 
General settings
Product size range:   - 
Primer optimum size:  
Primer max size:  
Primer min size:  
Primer optimum TM:  
Primer max TM:  
Primer min TM:  
Max TM diffrence:  
Primer lowercase masking:
e-PCR

Product size range :  -   
Primer max TM : 
Primer min TM : 
Concentration of monovalent cations : 
Concentration of divalent cations : 
Concentration of dNTPs(mM) : 
Annealing oligo concentration(nM) : 
History

Previous result is not found.

Target:
 :  -   
Flanking region (bp):

 
* Maximum size of  target + flanking region is 50,000 bp
Avoid sites around
the target (bp): 		 
Variant sites masking:
Note: Variant sites (regardless "hetero" or "homo") passing thresholds set below will be masked as "N" in the input sequence.
Quality: ≧ 
DEPTH: ≧ 

  Exporting FASTA
Chromosome:
Position:
  		 -    
* Maximum size is 200,000 bp
Variant Filer: Quality:  >=  
DEPTH:  >=  
Line break (bp):   =  
 * Use 0 for non-breaking.

Note: The fasta file being exported shows preferentially variant type allele regardless of its genotype "heterozygous or homozygous".
If GT is "1/2"(hetero without REF), the left side of the two alleles separated by comma is output.
Ns in the alignment indicates unmapped site (Depth = 0) as well as site below the threshold of depth and quality user set.

Chromosome:
Position:
  		 -  
    * Maximum size is 200,000 bp
ID:
Variant Filter: Quality:  >=  
DEPTH:  >=  

  • Accession list



  • Accession title

    • View
    ID or NAME   Subtitle   Width  
    Color
    Colors for each groups   Destination group   Color   ▓ 

  • Recovery list (original)

    •   Search
    Keyword  
    (eg. Os01g0100100-01) * The keyword must have at least 4 characters.

     


    Position  
    History
    : Quality of variant
    : Depth of all reads
    : Depth of alternative allele
    SnpEff Variant impact:
           

    To learn more about using TASUKE, visit this page.
  • Display Modes

    • Modes shown below can be changed at menu bar.
    SNP only
    SNP frequency is shown in blue gradient.
    Regions with no mapped reads are highlighted in yellow.
    DEPTH
    Average depth value of the block is shown in gray gradient.
    Depth & SNP
    SNP frequency is shown as inner box with the depth as background.
  • Mouse
    		•
    Drag Horizontally on the map.
    Double click to zoom in around the clicked point.
    Click on an accession name for changing the reference.
    Click again to restore the reference to the default setting.
  • Shortcut keys
    		•
     Movements 
    ← Move to left window
    → Move to right window
    <space> Move to right window
    <home> Move to head of current chr
    <end> Move to tail of current chr
    ↑ Zoom in
    <+> Zoom in
    <-> Zoom out
    ↓ Zoom out
     Display modes 
    p Show/Hide DEPTH
    s Show/Hide SNP
    i Show/Hide INDEL
    d Show/Hide indicator on map
    n Show/Hide DEPTH=0
    e On/Off SnpEff
    b On/Off Block mode
    h This manual page.
    Keyword:
    You will lose just all of your settings.
    (Data won't be deleted.)
    * It will be reloaded when restore.

    * It will be reloaded.
    : Number of blocks
    : Block Height
    : Block Width

  • ▓ MODIFIER
    • INTERGENIC
    • UPSTREAM
    • DOWNSTREAM
    • UTR_5_PRIME
    • UTR_3_PRIME
    • REGULATION
    • INTRAGENIC
    • GENE
    • INTRON_CONSERVED
    • INTRON
    • INTERGENIC_CONSERVED
  • ▓ LOW
    • SYNONYMOUS_START
    • NON_SYNONYMOUS_START
    • START_GAINED
    • SYNONYMOUS_CODING
    • SYNONYMOUS_STOP
    • EXON
    • CDS
    • TRANSCRIPT
    • CHROMOSOME_LARGE_DUPLICATION
    • SPLICE_SITE_REGION
    • SPLICE_SITE_BRANCH
    • SPLICE_SITE_BRANCH_U12
    • MICRO_RNA
    • NON_SYNONYMOUS_STOP
  • ▓ MODERATE
    • NON_SYNONYMOUS_CODING
    • CODON_CHANGE
    • CODON_INSERTION
    • CODON_CHANGE_PLUS_CODON_INSERTION
    • CODON_DELETION
    • CODON_CHANGE_PLUS_CODON_DELETION
    • UTR_5_DELETED
    • UTR_3_DELETED
    • CHROMOSOME_LARGE_INVERSION
    • NEXT_PROT
  • ▓ HIGH
    • SPLICE_SITE_ACCEPTOR
    • SPLICE_SITE_DONOR
    • START_LOST
    • EXON_DELETED
    • FRAME_SHIFT
    • STOP_GAINED
    • STOP_LOST
    • RARE_AMINO_ACID
    • CHROMOSOME_LARGEDELETION
    • EXON_DELETED_PARTIAL
    • EXON_DUPLICATION
    • EXON_DUPLICATION_PARTIAL
    • EXON_INVERSION
    • EXON_INVERSION_PARTIAL
    • GENE_DELETED
    • GENE_DUPLICATION
    • GENE_FUSION
    • GENE_FUSION_HALF
    • GENE_FUSION_REVERESE
    • GENE_REARRANGEMENT
    • PROTEIN_PROTEIN_INTERACTION_LOCUS
    • PROTEIN_STRUCTURAL_INTERACTION_LOCUS
    • TRANSCRIPT_DELETE
  • ▓ MODIFIER
    • intergenic_region
    • upstream_gene_variant
    • downstream_gene_variant
    • 5_prime_utr_variant
    • 3_prime_utr_variant
    • regulatory_region_variant
    • intragenic_variant
    • gene_variant
    • exon_variant
    • conserved_intron_variant
    • intron_variant
    • conserved_intergenic_variant
    • coding_sequence_variant
    • miRNA
    • non_coding_transcript_exon_variant
    • non_coding_transcript_variant
    • transcript_variant
    • sequence_feature
  • ▓ LOW
    • start_retained
    • initiator_codon_variant
    • synonymous_variant
    • stop_retained_variant
    • splice_region_variant
    • 5_prime_UTR_prematurestart_codon_gain_variant
    • 5_prime_UTR_premature_start_codon_gain_variant
    • start_codon_gain_variant
  • ▓ MODERATE
    • missense_variant
    • inframe_insertion
    • inframe_deletion
    • disruptive_inframe_insertion
    • disruptive_inframe_deletion
    • 5_prime_utr_truncation
    • 3_prime_utr_truncation
  • ▓ HIGH
    • splice_acceptor_variant
    • splice_donor_variant
    • start_lost
    • exon_loss_variant
    • frameshift_variant
    • stop_gained
    • stop_lost
    • rare_amino_acid_variant
    • duplication
    • inversion
    • feature_ablation
    • gene_fusion
    • bidirectional_gene_fusion
    • rearranged_at_DNA_level
    • protein_protein_contact
    • structural_interaction_variant
    • chromosome

    • What is SnpEff?
      GWAS
    Select phenotype
    Select
    chromosome
    Filter by SnpEff Impact level
    Sort by