PRIMER

using tool

primer3-

official site

Reference

Andreas Untergasser,Ioana Cutcutache,Triinu Koressaar,
Jian Ye,Brant C. Faircloth,Maido Remm,Steven G. Rozen
Primer3-new capabilities and interfaces
Nucleic Acids Res (2012) 40 (15): e115.
https://academic.oup.com/nar/article/40/15/e115/1223759/Primer3-new-capabilities-and-interfaces

ePCR

using tool

MFEprimer-
TASUKE+ only supports MFEprimer-, not MFEprimer-3.0+.

official site

Reference

Wubin Qu, Yang Zhou, Yanchun Zhang, Yiming Lu,
Xiaolei Wang, Dongsheng Zhao,Yi Yang,Chenggang Zhang
MFEprimer-2.0: a fast thermodynamics-based program for checking PCR primer specificity
Nucleic Acids Res (2012) 40 (W1): W205-W208
https://academic.oup.com/nar/article/40/W1/W205/1751577/MFEprimer-2-0-a-fast-thermodynamics-based-program