PRIMER

using tool

primer3-

official site

Reference

Andreas Untergasser,Ioana Cutcutache,Triinu Koressaar,

Jian Ye,Brant C. Faircloth,Maido Remm,Steven G. Rozen

Primer3-new capabilities and interfaces

Nucleic Acids Res (2012) 40 (15): e115.

https://academic.oup.com/nar/article/40/15/e115/1223759/Primer3-new-capabilities-and-interfaces

---

ePCR

using tool

MFEprimer-

TASUKE+ supports both MFEprimer-2 and MFEprimer-3.

official site

Reference

Kun Wang, Haiwei Li, Yue Xu, Qianzhi Shao, Jianming Yi, Ruichao Wang,

Wanshi Cai, Xingyi Hang, Chenggang Zhang, Haoyang Cai, Wubin Qu

MFEprimer-3.0: quality control for PCR primers.

Nucleic Acids Res (2019) 47(W1): W610-W613

https://academic.oup.com/nar/article/47/W1/W610/5486745