
- Installation.

0) Unpacking
 # tar xvzf tasuke_sample_data.tar.gz
 # cd ./tasuke_sample_data


1) Initialization

 # tasuke_init.pl -db [db_name] -u [user_name] -p [password]

   : Input chromosome's information

   > Where is the csv file? # reference.csv
   > Are you sure creating database?[y|n] y
   
   
2) Add accession
  
 # tasuke_accession.pl -db [db_name] -u [user_name] -p [password]
  
  : Input list of accessions

   > Where is the csv file? # accession.csv
   > Are you sure adding or updating database?[y|n] > y
  
  
3) Input reference(.fa)

 # tasuke_ref.pl -f ./reference.fa -db [db_name] -u [user_name] -p [password]


4) Input variant(.vcf)

  # tasuuke_variant_vcf.pl -n list01 -f ./variants/list01.vcf -t samtools -db [db_name] -u [user_name] -p [password]
  ..
  # tasuuke_variant_vcf.pl -n list10 -f ./variants/list01.vcf -t samtools -db [db_name] -u [user_name] -p [password]

  
5) Input depth(.depth)

 # tasuke_tsv_db.pl -n list01 -f list01.tsv -db [db_name] -u [user_name] -p [password]
 ..
 # tasuke_tsv_db.pl -n list10 -f list10.tsv -db [db_name] -u [user_name] -p [password]
  
  
6) Input track(.gff)

 # tasuke_track_gff.pl -t transcript -f transcripts.gff -db [db_name] -u [user_name] -p [password]

